A gardener wants to find out whether spraying a new organic pesticide reduces the number of aphids (greenfly) on rose bushes, and whether it affects the number of ladybirds (which feed on aphids) living in the garden. She chooses two similar gardens, each with the same number and variety of rose bush, in a similar sunny position. Garden A is sprayed with the pesticide once a week; Garden B is left unsprayed as a comparison. Each month for three months, she counts the mean number of aphids and ladybirds per rose bush in each garden.
| Month: 1 | 2 | 3 |
|---|
| Garden A (sprayed), mean aphids per bush: 40 | 15 | 5 |
| Garden A (sprayed), mean ladybirds per bush: 6 | 3 | 1 |
| Garden B (unsprayed), mean aphids per bush: 42 | 58 | 70 |
| Garden B (unsprayed), mean ladybirds per bush: 5 | 9 | 14 |
(a)State two variables the gardener should keep the same between Garden A and Garden B, other than whether the pesticide is sprayed, to make this a fair comparison.(2)
(b)Describe what the data shows happened to the aphid population in each garden over the three months.(1)
(c)Explain, referring to the food chain between aphids and ladybirds, why the ladybird population also fell in Garden A, even though the pesticide was not sprayed directly onto the ladybirds.(1)
(d)Suggest one reason the gardener's pesticide, despite reducing aphid numbers, might still be considered harmful to the wider garden ecosystem.(1)
(e)Suggest one improvement to the investigation that would make its conclusion more reliable.(1)
(Total for Question 30 is 6 marks)